Individual cells or cell aggregates dispersed and growing in moving liquid media is known as suspension culture.Explants used in suspension cultureIn regular practice suspension culture is initiated by transferring piece of undifferentiated and friable cells to a liquid medium which is continuously agitated by a rotator shaker.Suspension cultures have also been started from sterile seedlings or imbibed embryos or leaves. Leaves and other tissues can be gently grinded using homogenizer. This homogenate containing living cells, dead cells and cell debris is cleared by filtration and centrifugation and then transferred to a liquid medium. This is the mechanical methodIn enzymatic method, pectinases (enzymes which digest pectin cell wall) are used for isolation of single cells.Method At first...
Posted in: 
